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| Registros recuperados : 13 | |
| 3. |  | NARANCIO, R.; JOHN, U.; MASON, J.; GIRALDO, P.; SPANGENBERG, G. Digital PCR (dPCR) and qPCR mediated determination of transgene copy number in the forage legume white clover (Trifolium repens). Molecular Biology Reports, 2021, volume 48, Issue 4, Pages 3069 - 3077. Doi: https://doi.org/10.1007/s11033-021-06354-5 Article history: Received, 03 February 2021; Accepted, 12 April 2021; Published, 16 April 2021.
This project was funded by Agriculture Victoria, and Dairy Australia.| Biblioteca(s): INIA Las Brujas. |
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| 4. | | NARANCIO, R.; JOHN, U.; MASON, J.; SPANGENBERG, G. Selection of optimal reference genes for quantitative RT-PCR transcript abundance analysis in white clover (Trifolium repens L.). Functional Plant Biology, 2018, Volume 45, Issue 7, Pages 737-744. Doi: https://doi.org/10.1071/FP17304 Article history: Received 1 November 2017; Accepted 21 January 2018; Published online 16 February 2018.
Corresponding author: John, U.; Agriculture Victoria Research, AgriBio Centre for AgriBioscience, La Trobe University, Melbourne,...| Biblioteca(s): INIA Las Brujas. |
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| 5. | | DALLA RIZZA, M.; DIAZ, P.; NARANCIO, R.; CABRERA, A.; FERREIRA, F. Biomolecules as host defense weapons against microbial pathogens. (Review) Recent Patents on DNA and Gene Sequences, 2008, v.2, no.2, p.82-96. Article history: Received: April 21, 2008 / Accepted: April 29, 2008 / Revised: April 29, 2008.| Biblioteca(s): INIA Las Brujas. |
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| 7. | | ALEM, D.; NARANCIO, R.; DIAZ, P.; REBUFFO, M.; ZARZA, R.; DALLA RIZZA, M. Molecular characterization of Lotus corniculatus cultivars using transferable microsatellite markers. Ciencia e Investigación Agraria, Santiago de Chile, v. 38, n. 3, p. 463-471, 2011. Article history: Received September 7 2010. /Accepted June 21, 2011.| Biblioteca(s): INIA La Estanzuela. |
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| 8. | | NARANCIO, R.; ZORRILLA, P.; ROBELLO, C.; GONZÁLEZ-ARCOS, M.; VILARÓ, F.; PRITSCH, C.; DALLA RIZZA, M. Insights on gene expression response of a characterized resistant genotype of Solanum commersonii Dun. against Ralstonia solanacearum. European Journal of Plant Pathology, 2013, v.136, no.4, p.823-835. Article history: Accepted: 27 March 2013 / Published online: 28 April 2013.| Biblioteca(s): INIA Las Brujas. |
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| 9. | | REAL, D.; REYNO, R.; DALLA RIZZA, M.; ALTIER, N.; DO CANTO, J.; NARANCIO, R.; FOLLE, G.; BURGUEÑO, J. Caracterización de genotipos de Paspalum notatum por tolerancia a bajas temperaturas y reacción frente a Claviceps paspali ln: Reunión del grupo técnico en forrajeras del Cono Sur, 22., 2008, Minas, Uruguay Bioma campos: innovando para mantener su sustentabilidad y competitividad. Memorias. Minas (Uruguay): INIA; FAO; PROCISUR, 2008. p. 156 Versión impresa y en CD ROM Instituto Nacional de Investigación Agropecuaria, Uruguay; FAO; PROCISUR| Biblioteca(s): INIA Las Brujas; INIA Tacuarembó. |
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| 10. | | NARANCIO, R.; DING, Y.L.; LIN, Y.H.; SAHAB, S.; PANTER, S.; HAYES, M.; JOHN, U.; MASON, J.; SPANGENBERG, G. Generación y caracterización molecular y funcional de plantas de trébol blanco transgénicas con las características de senescencia retardada, tolerancia a aluminio y resistencia a Alfalfa Mosaic Virus (AMV). [resumen] In: INIA (Instituto Nacional de Investigación Agropecuaria); INIA Las Brujas; Biotecnología. Jornada de Agrobiotecnología, XI. Encuentro Nacional de REDBIO, III. Jornada técnica. Las Brujas, Canelones (UY): INIA, 2018. p.10-11. (Serie Actividades de Difusión; 786)| Biblioteca(s): INIA Las Brujas. |
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| 11. | | NARANCIO, R.; DING, Y.-L.; LIN, Y.-H.; SAHAB, S.; PANTER, S.; HAYES, M.; JOHN, U.; ANDERSON, H.; MASON, J.; SPANGENBERG, G. Application of linked and unlinked co transformation to generate triple stack, marker free, transgenic white clover (Trifolium repens L.). Plant Cell, Tissue and Organ Culture, 2020. DOI: https://doi.org/10.1007/s11240-020-01891-6 Article history: Received: 19 May 2020 / Accepted: 15 July 2020.| Biblioteca(s): INIA Las Brujas. |
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| 12. | | NARANCIO, R.; DING, Y.-L.; LIN, Y.-H.; SAHAB, S.; PANTER, S.; HAYES, M.; JOHN, U.; ANDERSON, H.; MASON, J.; SPANGENBERG, G. Correction to: Application of linked and unlinked co‑transformation to generate triple stack, marker‑free, transgenic white clover (Trifolium repens L.) (Plant Cell, Tissue and Organ Culture (PCTOC), (2020), 142, 3, (635-646), 10.1007/s11240-020-01891-6). Plant Cell, Tissue and Organ Culture, 2020. DOI: https://doi.org/10.1007/s11240-020-01891-6 Article history: Received: 19 May 2020 / Accepted: 15 July 2020.| Biblioteca(s): INIA Treinta y Tres. |
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| 13. | | REYNO, R.; NARANCIO, R.; SPERANZA, P.; DO CANTO, J.; LOPEZ-CARRO, B.; HERNANDEZ, P.; BURGUEÑO, J.; REAL, D.; DALLA RIZZA, M. Molecular and cytogenetic characterization of a collection of bahiagrass (Paspalum notatum Flügge) native to Uruguay. Genetic Resources and Crop Evolution, 2012, v. 59, no.8, p. 1823-1832. Article history: Received: 9 August 2011 / Accepted: 30 January 2012 / Published online: 22 February 2012.| Biblioteca(s): INIA Las Brujas; INIA Tacuarembó. |
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| Registros recuperados : 13 | |
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Registro completo
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Biblioteca (s) : |
INIA La Estanzuela. |
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Fecha actual : |
19/09/2014 |
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Actualizado : |
23/10/2019 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
B - 3 |
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Autor : |
ALEM, D.; NARANCIO, R.; DIAZ, P.; REBUFFO, M.; ZARZA, R.; DALLA RIZZA, M. |
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Afiliación : |
DIEGO MARTIN ALEM GLISON, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay; RAFAEL NARANCIO FERES, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay; PAOLA ALEXANDRA DIAZ DELLAVALLE, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay; MONICA IRENE REBUFFO GFELLER, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay; RODRIGO TABARE ZARZA FUENTES, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay; MARCO DALLA RIZZA VILARO, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay. |
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Título : |
Molecular characterization of Lotus corniculatus cultivars using transferable microsatellite markers. |
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Fecha de publicación : |
2011 |
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Fuente / Imprenta : |
Ciencia e Investigación Agraria, Santiago de Chile, v. 38, n. 3, p. 463-471, 2011. |
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ISSN : |
0304-5609 |
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DOI : |
10.4067/S0718-16202011000300015 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received September 7 2010. /Accepted June 21, 2011. |
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Contenido : |
Abstract :Lotus corniculatus L. is the most important agricultural species in the genus Lotus and is the most widely distributed Lotus species worldwide. L. corniculatus genotypes form complex groups that are difficult to recognize both morphologically and biochemically. Given the extensive and expensive process of isolating Simple Sequence Repeats (SSR, also called microsatellites), the possibility of using microsatellites already identified in related species is highly attractive. The aim of this work was the identification and validation of transferable microsatellite markers in L. corniculatus, and using those markers to study the genetic variability among four cultivars. Each cultivar of L. corniculatus was represented by 15 genotypes. Ten microsatellite markers were evaluated, and from those, four were selected based on their discriminative values observed among cultivars. We detected 29 alleles for the four markers, and there was an average of 7.25 alleles per locus. The marker TM0197 had the fewest number of alleles (5) and TM0083 had the highest number of alleles (10). The polymorphic information content (PIC) for the selected markers varied from 0.19 to 0.35, and the markers were therefore classified as highly informative. Based on the markers, we found high variability between individuals of the same cultivar. The use of transferable microsatellite markers could be useful to differentiate individuals at a relatively low cost, showing a great potential for use in breeding programs. MenosAbstract :Lotus corniculatus L. is the most important agricultural species in the genus Lotus and is the most widely distributed Lotus species worldwide. L. corniculatus genotypes form complex groups that are difficult to recognize both morphologically and biochemically. Given the extensive and expensive process of isolating Simple Sequence Repeats (SSR, also called microsatellites), the possibility of using microsatellites already identified in related species is highly attractive. The aim of this work was the identification and validation of transferable microsatellite markers in L. corniculatus, and using those markers to study the genetic variability among four cultivars. Each cultivar of L. corniculatus was represented by 15 genotypes. Ten microsatellite markers were evaluated, and from those, four were selected based on their discriminative values observed among cultivars. We detected 29 alleles for the four markers, and there was an average of 7.25 alleles per locus. The marker TM0197 had the fewest number of alleles (5) and TM0083 had the highest number of alleles (10). The polymorphic information content (PIC) for the selected markers varied from 0.19 to 0.35, and the markers were therefore classified as highly informative. Based on the markers, we found high variability between individuals of the same cultivar. The use of transferable microsatellite markers could be useful to differentiate individuals at a relatively low cost, showing a great potential for use in b... Presentar Todo |
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Palabras claves : |
GENETIC VARIABILITY; LOTUS JAPONICUS; MICROSATELLITES; SIMPLE SEQUENCE REPEATS; SSR; VARIABILIDAD GENÉTICA. |
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Thesagro : |
CULTIVOS. |
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Asunto categoría : |
F01 Cultivo |
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URL : |
http://www.scielo.cl/pdf/ciagr/v38n3/art15.pdf
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Marc : |
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008 2011 bl uuuu u00u1 u #d
022 $a0304-5609
024 7 $a10.4067/S0718-16202011000300015$2DOI
100 1 $aALEM, D.
245 $aMolecular characterization of Lotus corniculatus cultivars using transferable microsatellite markers.$h[electronic resource]
260 $c2011
500 $aArticle history: Received September 7 2010. /Accepted June 21, 2011.
520 $aAbstract :Lotus corniculatus L. is the most important agricultural species in the genus Lotus and is the most widely distributed Lotus species worldwide. L. corniculatus genotypes form complex groups that are difficult to recognize both morphologically and biochemically. Given the extensive and expensive process of isolating Simple Sequence Repeats (SSR, also called microsatellites), the possibility of using microsatellites already identified in related species is highly attractive. The aim of this work was the identification and validation of transferable microsatellite markers in L. corniculatus, and using those markers to study the genetic variability among four cultivars. Each cultivar of L. corniculatus was represented by 15 genotypes. Ten microsatellite markers were evaluated, and from those, four were selected based on their discriminative values observed among cultivars. We detected 29 alleles for the four markers, and there was an average of 7.25 alleles per locus. The marker TM0197 had the fewest number of alleles (5) and TM0083 had the highest number of alleles (10). The polymorphic information content (PIC) for the selected markers varied from 0.19 to 0.35, and the markers were therefore classified as highly informative. Based on the markers, we found high variability between individuals of the same cultivar. The use of transferable microsatellite markers could be useful to differentiate individuals at a relatively low cost, showing a great potential for use in breeding programs.
650 $aCULTIVOS
653 $aGENETIC VARIABILITY
653 $aLOTUS JAPONICUS
653 $aMICROSATELLITES
653 $aSIMPLE SEQUENCE REPEATS
653 $aSSR
653 $aVARIABILIDAD GENÉTICA
700 1 $aNARANCIO, R.
700 1 $aDIAZ, P.
700 1 $aREBUFFO, M.
700 1 $aZARZA, R.
700 1 $aDALLA RIZZA, M.
773 $tCiencia e Investigación Agraria, Santiago de Chile$gv. 38, n. 3, p. 463-471, 2011.
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